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mtnX [2019-07-31 13:07:23]
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mtnX [2019-07-31 13:07:23]

2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase
locus
BSU_13600
pI
4.66
mw
26.85 kDa
protein length
235 aa Sequence Blast
gene length
708 bp Sequence Blast
function
methionine salvage
product
2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase
essential
no
synonyms
ykrX
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.10|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine]

    • Gene

    Coordinates
    1,428,275 1,428,982

    The protein

    Catalyzed reaction/ biological activity

  • 2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate (according to Swiss-Prot)

    • Protein family

  • [SW|HAD superfamily] (according to UniProt)

    • Structure

  • [PDB|2FEA] [pubmed|17654724]

    • Expression and Regulation

    Operons

    genes
    [gene|42D2F9B958F62DFE447A1321E4109D676ECB899C|mtnW]-[gene|91633E6AF9A715166AE05BD7E540463DE352AA20|mtnX]-[gene|337F1E27C2E380DF8DCADE3581F74CFF52AFD224|mtnB]-[gene|C7D9E701B2CA2C9E85D69CAD6E0A45BE590BAED9|mtnD]
    description
    [Pubmed|12022921]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12022921], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [regulon|S-box|S-box]: termination, the [SW|S-box] [SW|riboswitch] binds S-adenosylmethionine resulting in termination [Pubmed|10094622], in [regulon|S-box|S-box]

    • regulation

  • induced by methionine starvation ([SW|S-box]) [Pubmed|10094622]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A785 (ykrX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/785 NBRP B. subtilis, Japan]
  • BKE13600 ([gene|91633E6AF9A715166AE05BD7E540463DE352AA20|mtnX]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATAATAAAAGGTTTTCGAG, downstream forward: _UP4_GAGAATGTAAAGGAGGTACA
  • BKK13600 ([gene|91633E6AF9A715166AE05BD7E540463DE352AA20|mtnX]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATAATAAAAGGTTTTCGAG, downstream forward: _UP4_GAGAATGTAAAGGAGGTACA

    • References

  • 12022921,11914366,12107147,15102328,12787499,18039762,17654724